Add AjaxControlToolkit and AjaxExtensionsToolbox to the references,

Modify your web.config file

<controls>
        <add tagPrefix=”ajaxToolkit” assembly=”AjaxControlToolkit” namespace=”AjaxControlToolkit”/>
    <add tagPrefix=”asp” namespace=”System.Web.UI” assembly=”System.Web.Extensions, Version=3.5.0.0, Culture=neutral, PublicKeyToken=31BF3856AD364E35″/>
    <add tagPrefix=”asp” namespace=”System.Web.UI.WebControls” assembly=”System.Web.Extensions, Version=3.5.0.0, Culture=neutral, PublicKeyToken=31BF3856AD364E35″/>
   </controls>

Add

<asp:ScriptManager ID=”scriptManager” runat=”server” EnablePartialRendering=”true” EnablePageMethods=”true”>
    </asp:ScriptManager>

under your FORM tag

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